Mária Lazarovská

Address

• Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia

E-mail

• lazarom@saske.sk

Phone

• +421-55-633 14 11 – 13

Technical Skills

• Mouse handling - collecting blood; drug administration- per os, subcutaneously, intramuscularly, intraperitoneally; dissection
• Ascaris suum, Echinococus multilocularis -parasite isolation (eggs, protoscoleces) and preparation of infectious cultures
• Techniques to obtain immune cells – isolation of peripheral lymphocytes by density gradient, isolation of macrophages by peritoneal lavage, splenocytes by spleen homogenization, isolation of small intestinal intraepithelial and lamina propria lymphocytes
• Methods – Lymphocyte proliferation by MTT test, protein detection, in vitro production of superoxide anion by macrophages, phenotyping of immune cells by monoclonal antibodies for flow cytometry, indirect ELISA, capture ELISA